Sperm chromatin structure assay (SCSA) parameters are related to fertilization, blastocyst development and ongoing pregnancy in IVF and ICSI cycles

Workflow

1. Spermatozoa immersion in agarose microgel and spread out on slide

2. Sample treatment with acid denaturation and lysis solution

3. Dehydration, stain and microscopic visualization

DNA fragmentation dynamics allows the assessment of cryptic sperm damage in human: evaluation of exposure to ionizing radiation, hyperthermia, acidic pH and nitric oxide.

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